Dr Steven Magennis
- Reader in Physical Chemistry (School of Chemistry)
telephone:
+44 (0) 141 330 7339
email:
Steven.Magennis@glasgow.ac.uk
pronouns:
He/him/his
C5-19, School of Chemistry, Joseph Black Building, Glasgow, G12 8QQ
Research interests
Research Interests
We use and develop single-molecule fluorescence techniques to probe the structure, dynamics and reactivity of DNA.
Details of the group's research can be found here.
Research groups
Publications
2024
Cadden, G. M., Wilken, S. J. and Magennis, S. W. (2024) A single CAA interrupt in a DNA three-way junction containing a CAG repeat hairpin results in parity-dependent trapping. Nucleic Acids Research, 52(15), pp. 9317-9327. (doi: 10.1093/nar/gkae644) (PMID:39041420) (PMCID:PMC11347167)
Ploetz, E. et al. (2024) A new twist on PIFE: photoisomerisation-related fluorescence enhancement. Methods and Applications in Fluorescence, 12(1), 012001. (doi: 10.1088/2050-6120/acfb58) (PMID:37726007) (PMCID:PMC10570931)
2023
Dodds, A. C., Sansom, H. G., Magennis, S. W. and Sutherland, A. (2023) Synthesis of Thiazoloindole α‑Amino Acids: Chromophores Amenable to One- and Two-Photon Induced Fluorescence. Organic Letters, 25(49), pp. 8942-8946. (doi: 10.1021/acs.orglett.3c03851) (PMID:38055619) (PMCID:PMC10729019)
Nobis, D., Sansom, H. G. and Magennis, S. W. (2023) Pulse-shaped broadband multiphoton excitation for single-molecule fluorescence detection in the far field. Methods and Applications in Fluorescence, 11(1), 017001. (doi: 10.1088/2050-6120/aca87f)
2022
Bianco, S. , Hu, T., Henrich, O. and Magennis, S. W. (2022) Heterogeneous migration routes of DNA triplet repeat slip-outs. Biophysical Reports, 2(3), 100070. (doi: 10.1016/j.bpr.2022.100070) (PMID:36299495) (PMCID:PMC9586884)
2021
Samaan, G. N., Wyllie, M. K., Cizmic, J. M., Needham, L.-M., Nobis, D., Ngo, K., Andersen, S., Magennis, S. W. , Lee, S. F. and Purse, B. W. (2021) Single-molecule fluorescence detection of a tricyclic nucleoside analogue. Chemical Science, 12(7), pp. 2623-2628. (doi: 10.1039/D0SC03903A) (PMID:34164030) (PMCID:PMC8179283)
Hu, T., Morten, M. J. and Magennis, S. M. (2021) Conformational and migrational dynamics of slipped-strand DNA three-way junctions containing trinucleotide repeats. Nature Communications, 12, 204. (doi: 10.1038/s41467-020-20426-3) (PMID:33420051) (PMCID:PMC7794359)
2020
Morten, M. J., Steinmark, E. and Magennis, S. W. (2020) Probing DNA dynamics: stacking-induced fluorescence increase (SIFI) versus FRET. ChemPhotoChem, 4(9), pp. 664-667. (doi: 10.1002/cptc.202000069)
Berrocal-Martin, R., Sanchez-Cano, C., Chiu, C. K.C., Needham, R. J., Sadler, P. J. and Magennis, S. W. (2020) Metallation‐induced heterogeneous dynamics of DNA revealed by single‐molecule FRET. Chemistry: A European Journal, 26(22), pp. 4980-4987. (doi: 10.1002/chem.202000458) (PMID:31999015)
Berrones‐Reyes, J. C., Muñoz‐Flores, B. M., Uscanga‐Palomeque, A. C., Santillán, R., Del Angel‐Mosqueda, C., Nobis, D., Cochrane, M. A., Magennis, S. and Jiménez‐Pérez, V. M. (2020) Two‐photon detection of organotin Schiff base complexes in cancer cells. ChemistrySelect, 5(5), pp. 1623-1627. (doi: 10.1002/slct.201904816)
Bell, J. D., Harkiss, A. H., Nobis, D., Malcolm, E., Knuhtsen, A., Wellaway, C. R., Jamieson, A. G. , Magennis, S. W. and Sutherland, A. (2020) Conformationally rigid pyrazoloquinazoline α-amino acids: one- and two-photon induced fluorescence. Chemical Communications, 56(12), pp. 1887-1890. (doi: 10.1039/C9CC09064A) (PMID:31956866)
2019
Nobis, D., Fisher, R. S., Simmermacher, M., Hopkins, P. A., Tor, Y., Jones, A. C. and Magennis, S. W. (2019) Single-molecule detection of a fluorescent nucleobase analog via multiphoton excitation. Journal of Physical Chemistry Letters, 10(17), pp. 5008-5012. (doi: 10.1021/acs.jpclett.9b02108) (PMID:31397575)
2018
Morten, M. J., Lopez, S. G., Steinmark, I. E., Rafferty, A. and Magennis, S. W. (2018) Stacking-induced fluorescence increase reveals allosteric interactions through DNA. Nucleic Acids Research, 46(21), pp. 11618-11626. (doi: 10.1093/nar/gky887) (PMID:30277520) (PMCID:PMC6265455)
Bood, M. et al. (2018) Pentacyclic adenine: a versatile and exceptionally bright fluorescent DNA base analog. Chemical Science, 9(14), pp. 3494-3502. (doi: 10.1039/C7SC05448C) (PMID:29780479) (PMCID:PMC5934695)
Baltierra Jasso, L., Morten, M. and Magennis, S. W. (2018) Sub-ensemble monitoring of DNA strand displacement using multiparameter single-molecule FRET. ChemPhysChem, 19(5), pp. 551-555. (doi: 10.1002/cphc.201800012) (PMID:29316151)
Fisher, R. S., Nobis, D., Füchtbauer, A. F., Bood, M., Grøtli, M., Wilhelmsson, L. M., Jones, A. C. and Magennis, S. W. (2018) Pulse-shaped two-photon excitation of a fluorescent base analogue approaches single-molecule sensitivity. Physical Chemistry Chemical Physics, 20(45), pp. 28487-28498. (doi: 10.1039/c8cp05496g) (PMID:30412214)
2017
Venkatesh, V. et al. (2017) Mitochondria-targeted spin-labelled luminescent iridium anticancer complexes. Chemical Science, 8(12), pp. 8271-8278. (doi: 10.1039/C7SC03216A)
Magennis, S. , Toulmin, A., Baltierra-Jasso, L. E., Morten, M. J., Sabir, T., McGlynn, P., Schröder, G. F., Smith, B. O. and Magennis, S. W. (2017) Conformational heterogeneity in a fully-complementary DNA three-way junction with a GC-rich branchpoint. Biochemistry, 56(37), pp. 4985-4991. (doi: 10.1021/acs.biochem.7b00677) (PMID:28820590)
Quinn, S. D. and Magennis, S. W. (2017) Optical detection of gadolinium(III) ions via quantum dot aggregation. RSC Advances, 7(40), pp. 24730-24735. (doi: 10.1039/C7RA03969G) (PMID:29308186) (PMCID:PMC5735353)
2016
Dalton, C. E., Quinn, S. D. , Rafferty, A., Morten, M. J., Gardiner, J. M. and Magennis, S. W. (2016) Single-molecule fluorescence detection of a synthetic heparan sulfate disaccharide. ChemPhysChem, 17(21), pp. 3442-3446. (doi: 10.1002/cphc.201600750) (PMID:27538128) (PMCID:PMC5111599)
Quinn, S. D. , Rafferty, A., Dick, E., Morten, M. J., Kettles, F. J., Knox, C., Murrie, M. and Magennis, S. W. (2016) Surface charge control of quantum dot blinking. Journal of Physical Chemistry C, 120(34), pp. 19487-19491. (doi: 10.1021/acs.jpcc.6b07779)
Baltierra-Jasso, L. E., Morten, M., Laflör, L., Quinn, S. D. and Magennis, S. W. (2016) DNA hairpin dynamics under molecular crowding conditions. Biophysical Journal, 110(3 S1), 634a-635a. (doi: 10.1016/j.bpj.2015.11.3397)
Quinn, S. D. , Dalton, C. E., Jeanneret, R. A., Gardiner, J. M. and Magennis, S. W. (2016) Single-molecule studies of fluorescently-labelled polysaccharides. Biophysical Journal, 110(3 S1), 636a-636a.
2015
Baltierra-Jasso, L. E., Morten, M. J., Laflor, L., Quinn, S. D. and Magennis, S. W. (2015) Crowding-induced hybridization of single DNA hairpins. Journal of the American Chemical Society, 137(51), pp. 16020-16023. (doi: 10.1021/jacs.5b11829) (PMID:26654490)
2014
Lane, R. S. K., Jones, R., Sinkeldam, R. W., Tor, Y. and Magennis, S. W. (2014) Two-photon-induced fluorescence of isomorphic nucleobase analogs. ChemPhysChem, 15(5), pp. 867-871. (doi: 10.1002/cphc.201400031)
2013
Pudney, C.R., Lane, R.S.K., Fielding, A.J., Magennis, S.W. , Hay, S. and Scrutton, N.S. (2013) Enzymatic single-molecule kinetic isotope effects. Journal of the American Chemical Society, 135(10), pp. 3855-3864. (doi: 10.1021/ja309286r)
2012
Sabir, T., Toulmin, A., Ma, L., Jones, A.C., McGlynn, P., Schröder, G.F. and Magennis, S.W. (2012) Branchpoint expansion in a fully complementary three-way DNA junction. Journal of the American Chemical Society, 134(14), pp. 6280-6285. (doi: 10.1021/ja211802z)
2011
Sabir, T., Schröder, G.F., Toulmin, A., McGlynn, P. and Magennis, S.W. (2011) Global structure of forked DNA in solution revealed by high-resolution single-molecule FRET. Journal of the American Chemical Society, 133(5), pp. 1188-1191. (doi: 10.1021/ja108626w)
2008
Channon, K.J., Devlin, G.L., Magennis, S.W. , Finlayson, C.E., Tickler, A.K., Silva, C. and MacPhee, C.E. (2008) Modification of fluorophore photophysics through peptide-driven self-assembly. Journal of the American Chemical Society, 130(16), pp. 5487-5491. (doi: 10.1021/ja710310c)
Articles
Cadden, G. M., Wilken, S. J. and Magennis, S. W. (2024) A single CAA interrupt in a DNA three-way junction containing a CAG repeat hairpin results in parity-dependent trapping. Nucleic Acids Research, 52(15), pp. 9317-9327. (doi: 10.1093/nar/gkae644) (PMID:39041420) (PMCID:PMC11347167)
Ploetz, E. et al. (2024) A new twist on PIFE: photoisomerisation-related fluorescence enhancement. Methods and Applications in Fluorescence, 12(1), 012001. (doi: 10.1088/2050-6120/acfb58) (PMID:37726007) (PMCID:PMC10570931)
Dodds, A. C., Sansom, H. G., Magennis, S. W. and Sutherland, A. (2023) Synthesis of Thiazoloindole α‑Amino Acids: Chromophores Amenable to One- and Two-Photon Induced Fluorescence. Organic Letters, 25(49), pp. 8942-8946. (doi: 10.1021/acs.orglett.3c03851) (PMID:38055619) (PMCID:PMC10729019)
Nobis, D., Sansom, H. G. and Magennis, S. W. (2023) Pulse-shaped broadband multiphoton excitation for single-molecule fluorescence detection in the far field. Methods and Applications in Fluorescence, 11(1), 017001. (doi: 10.1088/2050-6120/aca87f)
Bianco, S. , Hu, T., Henrich, O. and Magennis, S. W. (2022) Heterogeneous migration routes of DNA triplet repeat slip-outs. Biophysical Reports, 2(3), 100070. (doi: 10.1016/j.bpr.2022.100070) (PMID:36299495) (PMCID:PMC9586884)
Samaan, G. N., Wyllie, M. K., Cizmic, J. M., Needham, L.-M., Nobis, D., Ngo, K., Andersen, S., Magennis, S. W. , Lee, S. F. and Purse, B. W. (2021) Single-molecule fluorescence detection of a tricyclic nucleoside analogue. Chemical Science, 12(7), pp. 2623-2628. (doi: 10.1039/D0SC03903A) (PMID:34164030) (PMCID:PMC8179283)
Hu, T., Morten, M. J. and Magennis, S. M. (2021) Conformational and migrational dynamics of slipped-strand DNA three-way junctions containing trinucleotide repeats. Nature Communications, 12, 204. (doi: 10.1038/s41467-020-20426-3) (PMID:33420051) (PMCID:PMC7794359)
Morten, M. J., Steinmark, E. and Magennis, S. W. (2020) Probing DNA dynamics: stacking-induced fluorescence increase (SIFI) versus FRET. ChemPhotoChem, 4(9), pp. 664-667. (doi: 10.1002/cptc.202000069)
Berrocal-Martin, R., Sanchez-Cano, C., Chiu, C. K.C., Needham, R. J., Sadler, P. J. and Magennis, S. W. (2020) Metallation‐induced heterogeneous dynamics of DNA revealed by single‐molecule FRET. Chemistry: A European Journal, 26(22), pp. 4980-4987. (doi: 10.1002/chem.202000458) (PMID:31999015)
Berrones‐Reyes, J. C., Muñoz‐Flores, B. M., Uscanga‐Palomeque, A. C., Santillán, R., Del Angel‐Mosqueda, C., Nobis, D., Cochrane, M. A., Magennis, S. and Jiménez‐Pérez, V. M. (2020) Two‐photon detection of organotin Schiff base complexes in cancer cells. ChemistrySelect, 5(5), pp. 1623-1627. (doi: 10.1002/slct.201904816)
Bell, J. D., Harkiss, A. H., Nobis, D., Malcolm, E., Knuhtsen, A., Wellaway, C. R., Jamieson, A. G. , Magennis, S. W. and Sutherland, A. (2020) Conformationally rigid pyrazoloquinazoline α-amino acids: one- and two-photon induced fluorescence. Chemical Communications, 56(12), pp. 1887-1890. (doi: 10.1039/C9CC09064A) (PMID:31956866)
Nobis, D., Fisher, R. S., Simmermacher, M., Hopkins, P. A., Tor, Y., Jones, A. C. and Magennis, S. W. (2019) Single-molecule detection of a fluorescent nucleobase analog via multiphoton excitation. Journal of Physical Chemistry Letters, 10(17), pp. 5008-5012. (doi: 10.1021/acs.jpclett.9b02108) (PMID:31397575)
Morten, M. J., Lopez, S. G., Steinmark, I. E., Rafferty, A. and Magennis, S. W. (2018) Stacking-induced fluorescence increase reveals allosteric interactions through DNA. Nucleic Acids Research, 46(21), pp. 11618-11626. (doi: 10.1093/nar/gky887) (PMID:30277520) (PMCID:PMC6265455)
Bood, M. et al. (2018) Pentacyclic adenine: a versatile and exceptionally bright fluorescent DNA base analog. Chemical Science, 9(14), pp. 3494-3502. (doi: 10.1039/C7SC05448C) (PMID:29780479) (PMCID:PMC5934695)
Baltierra Jasso, L., Morten, M. and Magennis, S. W. (2018) Sub-ensemble monitoring of DNA strand displacement using multiparameter single-molecule FRET. ChemPhysChem, 19(5), pp. 551-555. (doi: 10.1002/cphc.201800012) (PMID:29316151)
Fisher, R. S., Nobis, D., Füchtbauer, A. F., Bood, M., Grøtli, M., Wilhelmsson, L. M., Jones, A. C. and Magennis, S. W. (2018) Pulse-shaped two-photon excitation of a fluorescent base analogue approaches single-molecule sensitivity. Physical Chemistry Chemical Physics, 20(45), pp. 28487-28498. (doi: 10.1039/c8cp05496g) (PMID:30412214)
Venkatesh, V. et al. (2017) Mitochondria-targeted spin-labelled luminescent iridium anticancer complexes. Chemical Science, 8(12), pp. 8271-8278. (doi: 10.1039/C7SC03216A)
Magennis, S. , Toulmin, A., Baltierra-Jasso, L. E., Morten, M. J., Sabir, T., McGlynn, P., Schröder, G. F., Smith, B. O. and Magennis, S. W. (2017) Conformational heterogeneity in a fully-complementary DNA three-way junction with a GC-rich branchpoint. Biochemistry, 56(37), pp. 4985-4991. (doi: 10.1021/acs.biochem.7b00677) (PMID:28820590)
Quinn, S. D. and Magennis, S. W. (2017) Optical detection of gadolinium(III) ions via quantum dot aggregation. RSC Advances, 7(40), pp. 24730-24735. (doi: 10.1039/C7RA03969G) (PMID:29308186) (PMCID:PMC5735353)
Dalton, C. E., Quinn, S. D. , Rafferty, A., Morten, M. J., Gardiner, J. M. and Magennis, S. W. (2016) Single-molecule fluorescence detection of a synthetic heparan sulfate disaccharide. ChemPhysChem, 17(21), pp. 3442-3446. (doi: 10.1002/cphc.201600750) (PMID:27538128) (PMCID:PMC5111599)
Quinn, S. D. , Rafferty, A., Dick, E., Morten, M. J., Kettles, F. J., Knox, C., Murrie, M. and Magennis, S. W. (2016) Surface charge control of quantum dot blinking. Journal of Physical Chemistry C, 120(34), pp. 19487-19491. (doi: 10.1021/acs.jpcc.6b07779)
Baltierra-Jasso, L. E., Morten, M., Laflör, L., Quinn, S. D. and Magennis, S. W. (2016) DNA hairpin dynamics under molecular crowding conditions. Biophysical Journal, 110(3 S1), 634a-635a. (doi: 10.1016/j.bpj.2015.11.3397)
Quinn, S. D. , Dalton, C. E., Jeanneret, R. A., Gardiner, J. M. and Magennis, S. W. (2016) Single-molecule studies of fluorescently-labelled polysaccharides. Biophysical Journal, 110(3 S1), 636a-636a.
Baltierra-Jasso, L. E., Morten, M. J., Laflor, L., Quinn, S. D. and Magennis, S. W. (2015) Crowding-induced hybridization of single DNA hairpins. Journal of the American Chemical Society, 137(51), pp. 16020-16023. (doi: 10.1021/jacs.5b11829) (PMID:26654490)
Lane, R. S. K., Jones, R., Sinkeldam, R. W., Tor, Y. and Magennis, S. W. (2014) Two-photon-induced fluorescence of isomorphic nucleobase analogs. ChemPhysChem, 15(5), pp. 867-871. (doi: 10.1002/cphc.201400031)
Pudney, C.R., Lane, R.S.K., Fielding, A.J., Magennis, S.W. , Hay, S. and Scrutton, N.S. (2013) Enzymatic single-molecule kinetic isotope effects. Journal of the American Chemical Society, 135(10), pp. 3855-3864. (doi: 10.1021/ja309286r)
Sabir, T., Toulmin, A., Ma, L., Jones, A.C., McGlynn, P., Schröder, G.F. and Magennis, S.W. (2012) Branchpoint expansion in a fully complementary three-way DNA junction. Journal of the American Chemical Society, 134(14), pp. 6280-6285. (doi: 10.1021/ja211802z)
Sabir, T., Schröder, G.F., Toulmin, A., McGlynn, P. and Magennis, S.W. (2011) Global structure of forked DNA in solution revealed by high-resolution single-molecule FRET. Journal of the American Chemical Society, 133(5), pp. 1188-1191. (doi: 10.1021/ja108626w)
Channon, K.J., Devlin, G.L., Magennis, S.W. , Finlayson, C.E., Tickler, A.K., Silva, C. and MacPhee, C.E. (2008) Modification of fluorophore photophysics through peptide-driven self-assembly. Journal of the American Chemical Society, 130(16), pp. 5487-5491. (doi: 10.1021/ja710310c)
Supervision
Research datasets
2024
Cadden, G., Schloetel, J.-G., McKenzie, G., Boocock, M., Magennis, S. and Stark, M. (2024) Direct observation of subunit rotation during DNA strand exchange by serine recombinases. [Data Collection]
Cadden, G., Wilken, S. and Magennis, S. (2024) A single CAA interrupt in a DNA three-way junction containing a CAG repeat hairpin results in parity-dependent trapping. [Data Collection]
2022
Magennis, S. and Heinrich, O. (2022) Heterogeneous migration routes of DNA triplet repeat slip-outs. [Data Collection]
2020
Hu, T., Morten, M. J. and Magennis, S. (2020) Conformational and migrational dynamics of slipped-strand DNA three-way junctions containing trinucleotide repeats. [Data Collection]
2019
Nobis, D., Fisher, R. S., Simmermacher, M., Hopkins, P. A., Tor, Y., Jones, A. C. and Magennis, S. (2019) Single-molecule detection of a fluorescent nucleobase analog via multiphoton excitation. [Data Collection]
2018
Morten, M. J., Lopez, S., Steinmark, I. E., Rafferty, A. and Magennis, S. (2018) Stacking-induced fluorescence increase reveals allosteric interactions through DNA. [Data Collection]